Incorporation of C14-pantothenate into the fatty acid synthetase complex of growing baker's yeast.
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Cytoplasmic Methionyl - tRNA Synthetase from Bakers ’ Yeast
Methionyl-tRNA synthetase has been purified from a yeast strain carrying the MESl structural gene on a high copy number plasmid (pFL1). The purified enzyme is a monomer of M, = 85,000 in contrast to its counterpart from Escherichia coli which is a dimer made up of identical subunits (M, = 76,000; Dardel, F., Fayat, G., and Blanquet, S . (1984) J. Bucteriol. 160,1115-1122). The yeast enzyme was ...
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A nutritional deficiency of fatty acids in young rats resulted in a marked elevation of the steady-state serum level of an injected dose of ol-aminoisclbutyric acid, and in a decrease in the transport of this model amino acid into skeletal muscle, heart, and intestine. The liver level was greatly increased. Adrenalectomy of the deficient rats reduced the uptake in both liver and kidney. These c...
متن کاملPurification and properties of the L-cysteinyl ribonucleic acid synthetase of bakers' yeast.
In this report a method is described for the puritication of the L-cystemyl-RNA synthetase of Bakers’ yeast. The enzyme, which was purified approximately 710-fold, was shown to have a molecular weight of approximately 160,000, and did not contain activity for any of the ammo acids commonly occurring in protein except L-cysteine. Optimum conditions for enzyme activity, such as substrate concentr...
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Previous studies on the physiological aspects of violacein biosynthesis (DeMoss and Evans, 1959) suggested that only L-tryptophan would serve as the primary source of carbon for pigment formation by Chromobacterium violaceum. In view of the pigment structure suggested by Ballantine et al. (1958), 5-[3-(5-hydroxyindolyl)]-3-(3-isatinyl)-2-pyrrolone, it seemed advisable to determine the actual so...
متن کاملThe incorporation of C14-orotic acid into nucleic acid pyrimidines in vitro.
The incorporation in vivo of compounds labeled with N15 or Cl4 into the purines and pyrimidines of nucleic acid has been repeatedly demonstrated (e.g. (l-3)). W e wish to report observations on the incorporation of radioactive erotic acid in vitro into the pyrimidines of the nucleic acid of rat liver slices. It is believed that this procedure will readily permit the study of precursors of nucle...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1966
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.56.2.633